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Construction of Hsp90β gene specific silencing plasmid and its transfection efficiency

JI Yewei, NIE Bin, LI Ping, ZHOU Yuanguo, XU Xiaoyu

Frontiers of Medicine 2007, Volume 1, Issue 3,   Pages 253-257 doi: 10.1007/s11684-007-0048-9

Abstract: efficiency of the plasmids in varying conditions of transfection.The transfection efficiency was measured by detection of enhanced green fluorescence protein (EGFP).After optimizing the ratio of plasmid to Lipofectamine, we achieved high transfection efficiency in HeK293Transfection efficiency was still low in the HepG2 cells.efficiency.

Keywords： HindIII     transfection efficiency     detection     enhanced     different    

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Effects of podocin transfection on CD2AP distribution in HEK293 cells

SHA Yugen, HUANG Songming, ZHANG Aihua, ZHAO Fei, CHEN Ronghua

Frontiers of Medicine 2008, Volume 2, Issue 1,   Pages 35-38 doi: 10.1007/s11684-008-0007-0

Abstract: this paper is to construct a podocin fluorescence expression vector and observe the effects of podocin transfectionAfter podocin transfection, CD2AP redistributed from the perinucleus to the cytoplasm in HEK293 cells

Keywords： pEGFP-NPHS2 expression     transfection     pEGFP-C2     cytoplasm     digested    

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Multifunctional peptide conjugated amphiphilic cationic copolymer for enhancing ECs targeting, penetrating and nuclear accumulation

Xinghong Duo, Lingchuang Bai, Jun Wang, Jintang Guo, Xiangkui Ren, Shihai Xia, Wencheng Zhang, Abraham Domb, Yakai Feng

Frontiers of Chemical Science and Engineering 2020, Volume 14, Issue 5,   Pages 889-901 doi: 10.1007/s11705-020-1919-8

Abstract: efficiency.The high internalization efficiency of these gene complexes was confirmed by flow cytometry.The results of transfection demonstrated that these multitargeting gene complexes possessed relativelyhigh transfection efficiency.efficiency has great potential in gene therapy.

Keywords： gene carriers     multitargeting function     ECs     transfection efficiency    

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Changes of phenotype and function of human CD4 CD25 T cells induced by transfection of Foxp3

WU Kui, BI Yutian, WANG Yaoli, WANG Changzheng

Frontiers of Medicine 2008, Volume 2, Issue 4,   Pages 366-369 doi: 10.1007/s11684-008-0070-6

Abstract: The aim of this paper is to explore the effects of transfection of Foxp3 gene on the phenotype and functionFoxp3-gene transfected CD4 T cells could express Foxp3 and transfection of Foxp3 gene up-regulated theAfter transfection, the proliferation of CD4 T cells was eliminated.
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Adenovirus-mediated tissue inhibitor of metalloproteinase-3 gene transfection inhibits rabbit intervertebral

Xudong YU MM, Zengwu SHAO MD, Liming XIONG MD, Weiwei XU MM, Hezhong WANG MM, Huifa XU MM,

Frontiers of Medicine 2009, Volume 3, Issue 4,   Pages 415-420 doi: 10.1007/s11684-009-0072-z

Abstract: The aim of this study was to investigate the inhibitory effects of recombinant adenovirus vector carrying tissue inhibitor of metalloproteinase-3 (RAdTIMP-3) against degeneration of rabbit intervertebral disc. Thirty Japanese white rabbits of 4 months old were randomly divided into 5 groups. Mild or moderate rabbit lumbar disc degeneration model was constructed with the controllable axial loading device by imposing 98N pressure at the discs for 2 weeks. Various doses of virus were injected into the degenerated discs as follows: 20μL of normal saline in group 1; 20μL of RAd66 (an empty adenovirus vector, 1.0×10OPU/mL) in group 2; and 20, 10, and 5μL of RAdTIMP-3 (1.0×10OPU/mL) in groups 3, 4, and 5, respectively. Two weeks after the injection, the discs were collected for investigations, including assessment of degeneration degrees according to the Thompson’s grading system, reverse-transcription polymerase chain reaction (RT-PCR) assay for TIMP-3 gene, Safranin O-Fast green staining, and immunohistochemical staining for TIMP-3 and type II collagen. According to Thompson’s criteria, the degeneration of groups 3, 4, and 5, especially group 3, was alleviated as compared with groups 1 and 2. RT-PCR revealed that the expression of TIMP-3 in groups 3, 4, and 5, especially in group 3, was significantly enhanced as compared with group 1 (<0.01). Both Safranin O-Fast green staining and type II collagen staining demonstrated better reserved integrity of disc matrix in groups 3, 4, and 5 than in groups 1 and 2. TIMP-3 staining exhibited an obvious increase of positive-staining rate in groups 3, 4, and 5 as compared with group 1. The positive-staining rate in group 3 (79.42%±1.35%) was about 3times that of group 1 (25.47%±5.46%, <0.01). RAdTIMP-3 can effectively protect the matrix of rabbit intervertebral disc against overloading-induced degeneration in a dose-dependent manner, resulting in the alleviation of disc degeneration.

Keywords： tissue inhibitor of metalloproteinase-3     intervertebral disc     rabbit     gene therapy    

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The effect of different agricultural management practices on irrigation efficiency, water use efficiency

La ZHUO, Arjen Y. HOEKSTRA

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 2,   Pages 185-194 doi: 10.15302/J-FASE-2017149

Abstract: This paper explores the effect of varying agricultural management practices on different water efficiencyindicators: irrigation efficiency (IE), crop water use efficiency (WUE), and green and blue water footprintImprovements in one water efficiency indicator may cause a decline in another.

Keywords： field management     irrigation efficiency     water footprint     water productivity     water use efficiency    

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Gene delivery into isolated

Nan Zheng, Ziyuan Song, Yang Liu, Lichen Yin, Jianjun Cheng

Frontiers of Chemical Science and Engineering 2017, Volume 11, Issue 4,   Pages 521-528 doi: 10.1007/s11705-017-1612-8

Abstract: The PPABLG was able to condense DNA to form nanocomplexes, and they exhibited significantly improved transfectionefficiencies compared with commercial transfection reagent Lipofectamine 2000 and classical cell penetrating

Keywords： α-helical polypeptide     plant gene delivery     protoplast     intact leaves     transfection    

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Evaluating R&D efficiency of China’s listed lithium battery enterprises

Frontiers of Engineering Management   Pages 473-485 doi: 10.1007/s42524-022-0213-5

Abstract: To guide future policies and understand proper ways of promoting R&D efficiency, we looked into the lithiumResults revealed little indication of a meaningful improvement in R&D efficiency throughout our samplewas 0.442, whereas the average pure technical efficiency was at 0.503, thus suggesting that they wereIn contrast, the performance of the five leading players seemed superior because their average efficiencyMoreover, they were experiencing increasing scale efficiency (IRS).

Keywords： Data Envelopment Analysis     R&D investment efficiency     China’s listed lithium battery enterprises     technicalefficiency     pure technical efficiency     scale efficiency    

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Improving the efficiency and effectiveness of global phosphorus use: focus on root and rhizosphere levels

Uwe LUDEWIG, Lixing YUAN, Günter NEUMANN

Frontiers of Agricultural Science and Engineering 2019, Volume 6, Issue 4,   Pages 357-365 doi: 10.15302/J-FASE-2019275

Abstract: Unfortunately, plant physiologists, agronomists, farmers and end users employ different measures for P use efficiency

Keywords： acquisition efficiency     plant growth promoting rhizobacteria     phosphate     use efficiency     utilization efficiency    

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Optimization of power and efficiency for an irreversible Diesel heat engine

Shiyan ZHENG, Guoxing LIN

Frontiers in Energy 2010, Volume 4, Issue 4,   Pages 560-565 doi: 10.1007/s11708-010-0018-9

Abstract: thermodynamic analysis and optimal control theory methods, the analytical expressions of power output and efficiencyMoreover, the optimum criterion of some important parameters, such as the power output, efficiency, pressure

Keywords： Diesel heat engine     irreversibility     power output     efficiency     parameter optimization    

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Methods and applications of DEA cross-efficiency: Review and future perspectives

Jie WU, Jiasen SUN, Liang LIANG

Frontiers of Engineering Management 2021, Volume 8, Issue 2,   Pages 199-211 doi: 10.1007/s42524-020-0133-1

Abstract: To address this problem, scholars have proposed the cross-efficiency evaluation (CREE) method.the theory and applications of CREE, including the non-uniqueness problem, the aggregation of cross-efficiency

Keywords： cross-efficiency evaluation     efficiency     secondary goal model     aggregation     review    

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STRATEGIES FOR IMPROVING WATER USE EFFICIENCY IN DRYLAND AGROECOSYSTEMS

Frontiers of Agricultural Science and Engineering 2021, Volume 8, Issue 4,   Pages 599-602 doi: 10.15302/J-FASE -2021409

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Effects of dwarfing genes on water use efficiency of bread wheat

Jiakun YAN, Suiqi ZHANG

Frontiers of Agricultural Science and Engineering 2017, Volume 4, Issue 2,   Pages 126-134 doi: 10.15302/J-FASE-2017134

Abstract: effects of different dwarfing genes on root growth, coleoptile length, grain yields and water using efficiency

Keywords： coleoptile length     wheat     dwarfing genes     grain yield     root     water use efficiency    

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neutrality potential of wastewater treatment plants: A novel evaluation framework integrating energy efficiency

Frontiers of Environmental Science & Engineering 2022, Volume 16, Issue 9, doi: 10.1007/s11783-022-1549-0

Abstract:

• Framework of indicators was established based on energy efficiency

Keywords： Wastewater treatment plants     Energy neutrality potential     Energy efficiency     Energy recovery     Evaluation    

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Approaches to achieve high grain yield and high resource use efficiency in rice

Jianchang YANG

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 2,   Pages 115-123 doi: 10.15302/J-FASE-2015055

Abstract: This article discusses approaches to simultaneously increase grain yield and resource use efficiencyare three important approaches to achieving the dual goal of high grain yield and high resource use efficiencyDeeper root distribution and higher leaf photosynthetic N use efficiency at lower N rates could be usedsink activity through increasing sugar-spikelet ratio at the heading time and enhancing the conversion efficiencyand non-flooded straw mulching cultivation, could substantially increase grain yield and water use efficiency

Keywords： rice     nitrogen-efficient cultivar     grain fill     harvest index     nitrogen use efficiency     water use efficiency    

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Title Author Date Type Operation

Construction of Hsp90β gene specific silencing plasmid and its transfection efficiency

JI Yewei, NIE Bin, LI Ping, ZHOU Yuanguo, XU Xiaoyu

Journal Article

Effects of podocin transfection on CD2AP distribution in HEK293 cells

SHA Yugen, HUANG Songming, ZHANG Aihua, ZHAO Fei, CHEN Ronghua

Journal Article

Multifunctional peptide conjugated amphiphilic cationic copolymer for enhancing ECs targeting, penetrating and nuclear accumulation

Xinghong Duo, Lingchuang Bai, Jun Wang, Jintang Guo, Xiangkui Ren, Shihai Xia, Wencheng Zhang, Abraham Domb, Yakai Feng

Journal Article

Changes of phenotype and function of human CD4 CD25 T cells induced by transfection of Foxp3

WU Kui, BI Yutian, WANG Yaoli, WANG Changzheng

Journal Article

Adenovirus-mediated tissue inhibitor of metalloproteinase-3 gene transfection inhibits rabbit intervertebral

Xudong YU MM, Zengwu SHAO MD, Liming XIONG MD, Weiwei XU MM, Hezhong WANG MM, Huifa XU MM,

Journal Article

The effect of different agricultural management practices on irrigation efficiency, water use efficiency

La ZHUO, Arjen Y. HOEKSTRA

Journal Article

Gene delivery into isolated

Nan Zheng, Ziyuan Song, Yang Liu, Lichen Yin, Jianjun Cheng

Journal Article

Evaluating R&D efficiency of China’s listed lithium battery enterprises

Journal Article

Improving the efficiency and effectiveness of global phosphorus use: focus on root and rhizosphere levels

Uwe LUDEWIG, Lixing YUAN, Günter NEUMANN

Journal Article

Optimization of power and efficiency for an irreversible Diesel heat engine

Shiyan ZHENG, Guoxing LIN

Journal Article

Methods and applications of DEA cross-efficiency: Review and future perspectives

Jie WU, Jiasen SUN, Liang LIANG

Journal Article

STRATEGIES FOR IMPROVING WATER USE EFFICIENCY IN DRYLAND AGROECOSYSTEMS

Journal Article

Effects of dwarfing genes on water use efficiency of bread wheat

Jiakun YAN, Suiqi ZHANG

Journal Article

neutrality potential of wastewater treatment plants: A novel evaluation framework integrating energy efficiency

Journal Article

Approaches to achieve high grain yield and high resource use efficiency in rice

Jianchang YANG

Journal Article